MATERIAL AND METHODS

The

proposed experiment “Organic, inorganic nitrogen management and beneficial

microbes for improving wheat productivity” will be carried out at Bacha Khan

Agricultural Research Farm (BARF), Bacha Khan University, Charsadda during

winter season 2017-18. The experiment will be laid out in a randomized complete

block design having three replications. Organic source of nutrient are Farm Yard

Manure {6 ton/ha} and Poultry manure {Poultry Manure 4 ton ha-1},

Inorganic source of nutrients {Nitrogen levels Control, 80, 120, 160 kg ha-1}

and beneficial microbes {with and without} will be tested in the experiment.

Nitrogen will be applied in split doses at sowing time and second irrigation

time. Size of the plot will be 1.8m x 4m. Row to row space of 30 cm will be

maintained. Seeds were planted at 120 kg ha-1 rate. Recommended dose

of Phosphorus at the rate of 80 kg ha-1 will be applied at sowing

time. All other agronomic practices were applied equally. Data will be recorded on days to emergence,

emergence m-2, number of tillers m-2, Days to anthesis,

Spike m-2, Non Productive tillers m-2, Grains spike-1,

Days to physiological maturity, Plant height (cm), Thousand grains weight (g),

Biological yield (kg ha-1), Grains yield (kg ha-1) and

Harvest index (%) will be recorded during the experiments.

Data will be

recorded on the following parameters

1. Days

to emergence

2. Emergence

m-2

3. Number

of tillers m-2

4. Days

to anthesis

5. Spikes

m-2

6. Non

productive tillers m-2

7. Grains

spike-1

8. Days

to physiological maturity

9. Plant

height (cm)

10. Thousand

grains weight (g)

11. Biological

yield (kg ha-1)

12. Grain

yield (kg ha-1)

13. Harvest

Index (%)

Days

to emergence

Data concerning

days to emergence will be recorded by counting the days taken from the date of

sowing to the date when 70-80% emergence completes in each sub plot.

Emergence

m-2

Data regarding emergence m-2

will be recorded by counting the number of seedlings in four central rows after

completion of emergence in each sub plot. The data will be converted to

emergence m-2 as:

Emergence m-2

= Total number of seedlings

emerged x 1

R-R distance x No. of rows x Row length

Tillers

m-2

Data on total

number of tillers in each sub plot will be taken by counting tillers in four

middle rows and then changed to tillers m-2

Tillers m-2

= Total number of tillers x 1

R-R distance x No. of rows x Row length

Days

to anthesis

Days to anthesis

data in each sub plot will be measured by counting days from sowing to the date

when 70-80% plants reached to anthesis phase.

Spikes

m-2

Spikes will be

counted in four central rows of each sub plot and then changed into spikes m-2

accordingly.

Spikes m-2

= Total number of tillers x 1

R-R distance x No. of rows x Row length

Non-productive

tillers m-2

Tillers having

no productive spikes will be counted in four middle rows in all sub plots and

changed accordingly to non-productive tillers m-2.

Grains

spike-1

For

measuring data on grains spike-1 ten spikes will be selected

randomly in each sub plot, threshed separately, counted number of grains of

each spikes and then averaged.

Days

to physiological maturity

Days will be

counted from sowing date to the date when 70 to 80% physiological maturity happened

in each sub plot.

Plant

height (cm)

Plant height

will be measured by measuring height of ten randomly selected plants from the

base of plant to the tip of spike in each sub plot and averaged.

Thousand

grains weight

Thousand grains

weight will be recorded by weighing thousand grains taken randomly from grain

lot of each sup plot.

Biological

yield (kg ha-1)

Data regarding

biological yield will be taken by reaping central four rows from every sub

plot, sun dried, weighed and then changed to kg ha-1 by using the formula:

Biological yield

(kg ha-1) = Biological

yield kg x 10000 m-2

ha-1

m(R-R) x row length cm x No. of

rows

Grain

yield (kg ha-1)

The harvested

four central rows in each sub plot will be threshed, cleaned, weighed and

changed into kg ha-1 by formula:

Grain yield (kg

ha-1) = ___Grain yield kg__________ x

10000 m-2 ha-1

M(R-R)

x row length cm x No. of rows

Harvest

index (%)

The following

formula was used for the calculation of harvest index:

Harvest index

(%) = ___Grain yield kg ha-1 x 100

Biological yield kg ha-1

Statistical

analysis

Method

applicable to the randomized complete block design (split-plot arrangement)

will be used for statistical analysis of the recorded data. For mean comparison

in case of significant difference least significant difference (LSD) test at 5%

level of significance will be used (Steel and Torrie, 1984).