MATERIAL data in each sub plot will be


proposed experiment “Organic, inorganic nitrogen management and beneficial
microbes for improving wheat productivity” will be carried out at Bacha Khan
Agricultural Research Farm (BARF), Bacha Khan University, Charsadda during
winter season 2017-18. The experiment will be laid out in a randomized complete
block design having three replications. Organic source of nutrient are Farm Yard
Manure {6 ton/ha} and Poultry manure {Poultry Manure 4 ton ha-1},
Inorganic source of nutrients {Nitrogen levels Control, 80, 120, 160 kg ha-1}
and beneficial microbes {with and without} will be tested in the experiment.
Nitrogen will be applied in split doses at sowing time and second irrigation
time. Size of the plot will be 1.8m x 4m. Row to row space of 30 cm will be
maintained. Seeds were planted at 120 kg ha-1 rate. Recommended dose
of Phosphorus at the rate of 80 kg ha-1 will be applied at sowing
time. All other agronomic practices were applied equally.  Data will be recorded on days to emergence,
emergence m-2, number of tillers m-2, Days to anthesis,
Spike m-2, Non Productive tillers m-2, Grains spike-1,
Days to physiological maturity, Plant height (cm), Thousand grains weight (g),
Biological yield (kg ha-1), Grains yield (kg ha-1) and
Harvest index (%) will be recorded during the experiments.

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Data will be
recorded on the following parameters

1.      Days
to emergence

2.      Emergence

3.      Number
of tillers m-2

4.      Days
to anthesis

5.      Spikes

6.      Non
productive tillers m-2

7.      Grains

8.      Days
to physiological maturity

9.      Plant
height (cm)

10.  Thousand
grains weight (g)

11.  Biological
yield (kg ha-1)

12.  Grain
yield (kg ha-1)

13.  Harvest
Index (%)



to emergence

Data concerning
days to emergence will be recorded by counting the days taken from the date of
sowing to the date when 70-80% emergence completes in each sub plot.


Data regarding emergence m-2
will be recorded by counting the number of seedlings in four central rows after
completion of emergence in each sub plot. The data will be converted to
emergence m-2 as:

Emergence m-2
=      Total number of seedlings
emerged    x 1

R-R distance x No. of rows x Row length


Data on total
number of tillers in each sub plot will be taken by counting tillers in four
middle rows and then changed to tillers m-2

Tillers m-2
=     Total number of tillers     x 1

R-R distance x No. of rows x Row length

to anthesis

Days to anthesis
data in each sub plot will be measured by counting days from sowing to the date
when 70-80% plants reached to anthesis phase.


Spikes will be
counted in four central rows of each sub plot and then changed into spikes m-2

Spikes m-2
=     Total number of tillers     x 1

R-R distance x No. of rows x Row length

tillers m-2

Tillers having
no productive spikes will be counted in four middle rows in all sub plots and
changed accordingly to non-productive tillers m-2.




measuring data on grains spike-1 ten spikes will be selected
randomly in each sub plot, threshed separately, counted number of grains of
each spikes and then averaged.



to physiological maturity

Days will be
counted from sowing date to the date when 70 to 80% physiological maturity happened
in each sub plot.

height (cm)

Plant height
will be measured by measuring height of ten randomly selected plants from the
base of plant to the tip of spike in each sub plot and averaged. 

grains weight

Thousand grains
weight will be recorded by weighing thousand grains taken randomly from grain
lot of each sup plot.

yield (kg ha-1)

Data regarding
biological yield will be taken by reaping central four rows from every sub
plot, sun dried, weighed and then changed to kg ha-1 by using the formula:

Biological yield
(kg ha-1) =             Biological
yield kg             x 10000 m-2

m(R-R) x row length cm x No. of

yield (kg ha-1)

The harvested
four central rows in each sub plot will be threshed, cleaned, weighed and
changed into kg ha-1 by formula:

Grain yield (kg
ha-1)    =          ___Grain yield kg__________   x  
10000 m-2 ha-1

x row length cm x No. of rows


index (%)

The following
formula was used for the calculation of harvest index: 

Harvest index
(%)       =          ___Grain yield kg ha-1      x 100

Biological yield kg ha-1     


applicable to the randomized complete block design (split-plot arrangement)
will be used for statistical analysis of the recorded data. For mean comparison
in case of significant difference least significant difference (LSD) test at 5%
level of significance will be used (Steel and Torrie, 1984).


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